FM Minimal medium w/o Glutamic acid and w/o Lysine Size:250g 2 at 25°C Suspend 67

2 at 25°C Suspend 67 gram powdered medium in 1 litre distilled water

Cold Spring harbour Laboratory

69 Store dry at room temperature

H2O) Clear and colourless solution pH (5%

vitamins and minerals essential for vigorous growth

FM Minimal medium w/o Glutamic acid and w/o Lysine Size:250g 2 at 25°C Suspend 67This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to