Yeast Potassium Nitrate Nitrogen Base Size:500g Tryptone 8 Yeast extract 5

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

2 at 25°C Suspend 13 gram powdered medium in 1 litre distilled water

AX Medium is used to induce and to cultivate an axenic culture of Dictyostelium discoideum from bacterially grown cells

Store dark

Suspend 25

Yeast Potassium Nitrate Nitrogen Base Size:500g Tryptone 8 Yeast extract 5Yeast Potassium Nitrate Nitrogen Base contains Potassium Nitrate as a sole nitrogen source to test nitrogen assimilation. The addition of a carbon source is required. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base. Yeasts which have grown on a rich medium like Yeast Agar may carry a reserve of nitrogen in the form of protein. To avoid errors due to this