Neidhardt Basal Salt Mixture, Glucose 2 g/L Size:447g (30L) These two newly developed media

These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies

7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element

Yeast extract 5 Dextrose 30 Adenine 0

15 g SP supplements (Ade

These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

Neidhardt Basal Salt Mixture, Glucose 2 g/L Size:447g (30L) These two newly developed mediaContaining all micro and macro salt, buffers and glucose. A mixture of Neidhardt basal salt mixture in a concentration of 14. 9 grams l with Neidhardt supplement mixture in a concentration of 3. 1 grams l. Suspend 14. 9 grams of Neidhardt basal salt mixture plus 3. 1 grams of Neidhardt supplement mixture in one litre of distilled water. Sterilisation by means of filtration. After dissolving a slight precipitation might occur. It is not clear what this